Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine

Cited:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 5H7

Concentration

1 mg/ml

View all EWSR1 Antibodies »

Product Specifications

Immunogen

Full length recombinant human EWSR1 expressed in and purified from E. coli. [UniProt# Q01844]

Localization

Nucleus. Cytoplasm. Cell membrane. Note: Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EWSR1 Antibody (5H7)

Western Blot: EWSR1 Antibody (5H7) [NBP1-92686]

Western Blot: EWSR1 Antibody (5H7) [NBP1-92686]

Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.
Immunocytochemistry/ Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686]

Immunocytochemistry/ Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686]

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686] - HeLa cell cultures stained with antibody NBP1-92686 (green) and chicken antibody to vimentin (NB300-223, red). Blue is a DNA stain, and it is clear that in these cells EWSR1 is localized along with the DNA in the nucleus.
Western Blot: EWSR1 Antibody (5H7) [NBP1-92686]

Western Blot: EWSR1 Antibody (5H7) [NBP1-92686]

Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Analysis of different cell line lysates using EWSR1 antibody, dilution 1:1000 (Green): [1] protein standard (Red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.

Applications for EWSR1 Antibody (5H7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000-1:2000
Application Notes
This EWSR1 (5H7) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where a band can be seen at approximately 80 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EWSR1

EWSR1 (Ewing sarcoma breakpoint region 1) belongs to TET family (Tls, EWSR1, TafII) of RNA-binding proteins and it is implicated in transcriptional regulation for specific genes, mRNA splicing and transcription initiation. EWSR1 has the potential to associate with basal transcription factor TFIID and RNA polymerase II complex, and is required for proper localization of aurora B during mitosis as well as for maintaining mitotic spindle integrity. EWSR1 also associates with EP300/CREBBP as coactivator and EWSR1-EP300/CREBBP facilitates FOS activation as well as HNF4 genes activation. EWSR1 activates other transcription factors such as POU4F1 (or BRN3A, 13q13) and POU5F1 (or OCT4, 6p21) which regulate differentiation of neuronal cells. EWSR1 and CCNL1 are also interacting partners of TFIP11 which is functionally related to spliceosome and involved in pre-mRNA splicing. EWSR1 is required for cell survival in CNS and performs important functions in regulating genomic integrity and in RNA/microRNA's maturation processes. EWSR1 is ubiquity expressed with dominant localization in the nucleus and rarely in cytoplasm or various subcellular compartments, depending on the methylation state of its RNA-binding domain. Several gene families, mainly encoding for transcriptional regulators, can translocate with EWSR1 and the chimeric proteins have an aberrant activity interfering with different molecular pathways crucial for cell growth, differentiation and proliferation. EWSR1 is involved in several sarcoma translocations and soft tissue myoepithelial tumors.

Long Name

RNA-binding protein EWS

Alternate Names

EWS, EWS oncogene

Entrez Gene IDs

2130 (Human)

Gene Symbol

EWSR1

UniProt

Q01844 (Human)

Additional EWSR1 Products

Product Documents for EWSR1 Antibody (5H7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EWSR1 Antibody (5H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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