Exosome Detection (Simple Western) Antibody Pack

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11742

Novus Biologicals, part of Bio-Techne
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NBP3-11742
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Key Product Details

Species

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Simple Western, Western Blot

Product Summary for Exosome Detection (Simple Western) Antibody Pack

This pack contains 1 vial each of: MAB50482-SP (25 ug); NBP2-67884 (100 uL); MAB1663-SP (25 ug); NB100-1965SS (0.025 ml)

Product Specifications

Specificity

Please see each vial's data sheet for specificity information specific to each product.

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Exosome Detection (Simple Western) Antibody Pack

Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]

Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]

Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 ug/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]

Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]

Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Kit Contents for Exosome Detection (Simple Western) Antibody Pack

Formulation, Preparation, and Storage

Formulation

See individual datasheets

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage is content dependent.

Background: Exosome Detection (Simple Western)

The Exosome Detection (Simple Western) Antibody Pack contains antibodies from each category recommended by the International Society for Extracellular Vesicles (ISEV) for characterization of extracellular vesicles (EVs). It includes one tetraspanin (CD63), one intracellular protein associated with multivesicular body (MVB) formation (TSG101), and one chaperone protein (HSP70). The pack also contains an antibody to determine the degree of contamination of exosome prep with secretory vesicles (Calnexin). All antibodies have been validation for use in Simple Western.

Additional Exosome Detection (Simple Western) Products

Product Documents for Exosome Detection (Simple Western) Antibody Pack

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Exosome Detection (Simple Western) Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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