Ezrin Antibody (3C12)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1310
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3C12
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
BALB/C mice were immunized with a recombinant fragment of human ezrin corresponding to amino acid 362-585.
Localization
Cell membrane
Specificity
This antibody reacts with a protein of 81 kDa. Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein, enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Ezrin Antibody (3C12)
![Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310]](https://resources.bio-techne.com/images/products/Ezrin-Antibody-3C12-Immunocytochemistry-Immunofluorescence-NB600-1310-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310]")
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310]
Immunocytochemistry/Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310] - Cultured Rat hippocampal neurons, courtesy of customer.![Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]](https://resources.bio-techne.com/images/products/Ezrin-Antibody-3C12-Immunohistochemistry-Paraffin-NB600-1310-img0004.jpg "Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]")
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded lung adenocarcinoma stained with Ezrin antibody (NB600-1310).![Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]](https://resources.bio-techne.com/images/products/Ezrin-Antibody-3C12-Immunohistochemistry-Paraffin-NB600-1310-img0001.jpg "Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]")
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310]
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded human lung stained with Ezrin antibody.Applications for Ezrin Antibody (3C12)
Application
Recommended Usage
Immunohistochemistry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:100-1:750
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
Reviewed Applications
Read 1 review rated 4 using NB600-1310 in the following applications:
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Ezrin
Alternate Names
CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2
Gene Symbol
EZR
Additional Ezrin Products
Product Documents for Ezrin Antibody (3C12)
Product Specific Notices for Ezrin Antibody (3C12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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