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Ezrin Antibody (CL2360) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44476

Novus Biologicals, part of Bio-Techne
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NBP3-44476

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2360

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P15311, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Ezrin Antibody (CL2360) - Azide and BSA Free

Ezrin Antibody (CL2360) - Azide and BSA Free Western Blot: Ezrin Antibody [NBP3-44476]

Western Blot: Ezrin Antibody [NBP3-44476]

Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
Ezrin Antibody (CL2360) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP3-44476]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP3-44476]

Staining of A-431 cells using the Anti-Ezrin monoclonal antibody) .
Ezrin Antibody (CL2360) - Azide and BSA Free Immunohistochemistry-Paraffin: Ezrin Antibody [NBP3-44476]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP3-44476]

Analysis in human placenta and skeletal muscle tissues using NBP3-44476 antibody. Corresponding Ezrin RNA-seq data are presented for the same tissues.

Applications for Ezrin Antibody (CL2360) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ezrin

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Gene Symbol

EZR

Additional Ezrin Products

Product Documents for Ezrin Antibody (CL2360) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ezrin Antibody (CL2360) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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