Ezrin Antibody (CL2384)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36760

Novus Biologicals, part of Bio-Techne
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NBP2-36760
NBP2-36760-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2384

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ezrin Antibodies »

Product Summary for Ezrin Antibody (CL2384)

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLE

Epitope

LSEQIQRALQLEEER

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ezrin Antibody (CL2384)

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in human small intestine and skeletal muscle tissues. Corresponding EZR RNA-seq data are presented for the same tissues.
Western Blot: Ezrin Antibody (CL2384) [NBP2-36760]

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760]

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Analysis of extracts from U-251 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-EZR monoclonal antibody.Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in U251 cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
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Applications for Ezrin Antibody (CL2384)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ezrin

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Gene Symbol

EZR

UniProt

P15311 (Human)

Additional Ezrin Products

Product Documents for Ezrin Antibody (CL2384)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ezrin Antibody (CL2384)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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