Ezrin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86863

Novus Biologicals, part of Bio-Techne
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NBP1-86863
NBP1-86863-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ezrin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ezrin Antibody

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863]

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining in human placenta and skeletal muscle tissues using anti-EZR antibody. Corresponding EZR RNA-seq data are presented for the same tissues.
Western Blot: Ezrin Antibody [NBP1-86863]

Western Blot: Ezrin Antibody [NBP1-86863]

Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: Ezrin Antibody [NBP1-86863]

Western Blot: Ezrin Antibody [NBP1-86863]

Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in human placenta tissue.
View 6 more images

Applications for Ezrin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ezrin

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Gene Symbol

EZR

Additional Ezrin Products

Product Documents for Ezrin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ezrin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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