F-Spondin/SPON1 Antibody (PSH02-84)

Western Blot: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352]

Western Blot: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Western blot analysis of F-Spondin/SPON1 on different lysates with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/1,000 dilution.

Lane 1: SW1990 cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: HepG2 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 91 kDa
Observed band size: 91120 kDa

Exposure time: 1 minutes 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32352) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352]

Immunocytochemistry/ Immunofluorescence: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Immunocytochemistry analysis of PANC-1 cells labeling F-Spondin/SPON1 with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352]

Flow Cytometry: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Flow cytometric analysis of PANC-1 cells labeling F-Spondin/SPON1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32352, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).