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FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79929

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # FABP5/3750

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human FABP5/E-FABP protein (Uniprot: Q01469)

Reactivity Notes

Others not known.

Localization

Nuclear, Cytoplasmic

Marker

Marker of Metastatic Potential in Colorectal Cancer

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

15.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79795).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free

Western Blot: FABP5/E-FABP Antibody (FABP5/3750)Azide and BSA Free [NBP2-79929]

Western Blot: FABP5/E-FABP Antibody (FABP5/3750)Azide and BSA Free [NBP2-79929]

Western Blot: FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free [NBP2-79929] - Western Blot Analysis of HeLa cell lysate using FABP5/E-FABP Antibody (FABP5/3750).
Immunohistochemistry-Paraffin: FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free [NBP2-79929]

Immunohistochemistry-Paraffin: FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free [NBP2-79929]

Immunohistochemistry-Paraffin: FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free [NBP2-79929] - Formalin-fixed, paraffin-embedded human Liver tissue stained with FABP5 Mouse Monoclonal Antibody (FABP5/3750).
Protein Array: FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free [NBP2-79929]

Protein Array: FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free [NBP2-79929]

Protein Array: FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free [NBP2-79929] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAbto its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Applications for FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

0.1 - 0.2 ug/mL

Immunohistochemistry-Paraffin

0.1 - 0.2 ug/mL

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: FABP5/E-FABP

Fatty Acid Binding Protein 5 encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

Long Name

Fatty Acid-Binding Protein 5

Alternate Names

E-FABP, EFABP

Gene Symbol

FABP5

Additional FABP5/E-FABP Products

Product Documents for FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FABP5/E-FABP Antibody (FABP5/3750) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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