FACA/FANCA Antibody (JE36-22)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32333
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE36-22
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human FACA/FANCA aa 1-150 / 1,455. (Uniprot: O15360)
Localization
Nucleus, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
163 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FACA/FANCA Antibody (JE36-22)
Western Blot: FACA/FANCA Antibody (JE36-22) [NBP3-32333]
Western Blot: FACA/FANCA Antibody (JE36-22) [NBP3-32333] - Western blot analysis of FACA/FANCA on different lysates with Rabbit anti-FACA/FANCA antibody (NBP3-32333) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: HT-29 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: K-562 cell lysate
Lane 9: Daudi cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 163 kDa
Observed band size: 163 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32333) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for FACA/FANCA Antibody (JE36-22)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FACA/FANCA
Defects in FANCA result in Fanconi Anemia, a genetically heterogeneous autosomal recessive disorder characterized by progressive pancytopenia, diverse congenital malformations, and a predisposition to develop malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. At the cellular level FANCA defects cause a hypersensitivity to DNA-damaging agents, chromosomal instability, and defective DNA repair.
Long Name
Fanconi Anemia, Complementation Group A
Alternate Names
FAA, FAH, FANCA, FANCH
Gene Symbol
FANCA
Additional FACA/FANCA Products
Product Documents for FACA/FANCA Antibody (JE36-22)
Product Specific Notices for FACA/FANCA Antibody (JE36-22)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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