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FACA/FANCA Antibody (JE36-22)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32333

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32333

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE36-22

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within Human FACA/FANCA aa 1-150 / 1,455. (Uniprot: O15360)

Localization

Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

163 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FACA/FANCA Antibody (JE36-22)

FACA/FANCA Antibody (JE36-22)

Western Blot: FACA/FANCA Antibody (JE36-22) [NBP3-32333]

Western Blot: FACA/FANCA Antibody (JE36-22) [NBP3-32333] - Western blot analysis of FACA/FANCA on different lysates with Rabbit anti-FACA/FANCA antibody (NBP3-32333) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: HT-29 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: K-562 cell lysate
Lane 9: Daudi cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 163 kDa
Observed band size: 163 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32333) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for FACA/FANCA Antibody (JE36-22)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FACA/FANCA

FANCA (Fanconi anemia group A) protein is a DNA repair protein that may function as a cell cycle checkpoint or in postreplication repair. FANCA may also be important for the maintenance of chromosome stability and reparation of interstrand DNA cross linkages.

Defects in FANCA result in Fanconi Anemia, a genetically heterogeneous autosomal recessive disorder characterized by progressive pancytopenia, diverse congenital malformations, and a predisposition to develop malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. At the cellular level FANCA defects cause a hypersensitivity to DNA-damaging agents, chromosomal instability, and defective DNA repair.

Long Name

Fanconi Anemia, Complementation Group A

Alternate Names

FAA, FAH, FANCA, FANCH

Gene Symbol

FANCA

Additional FACA/FANCA Products

Product Documents for FACA/FANCA Antibody (JE36-22)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FACA/FANCA Antibody (JE36-22)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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