Factor IX Antibody (2C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00002158-M01

Novus Biologicals, part of Bio-Techne
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H00002158-M01
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Coagulation Factor IX Antibodies »

Product Specifications

Immunogen

F9 (NP_000124, 96 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLT

Specificity

F9 - coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Factor IX Antibody (2C9)

Western Blot: Factor IX Antibody (2C9) [H00002158-M01]

Western Blot: Factor IX Antibody (2C9) [H00002158-M01]

Western Blot: Factor IX Antibody (2C9) [H00002158-M01] - Analysis of F9 expression in transfected 293T cell line by F9 monoclonal antibody (M01), clone 2C9.Lane 1: F9 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Western Blot: Factor IX Antibody (2C9) [H00002158-M01]

Western Blot: Factor IX Antibody (2C9) [H00002158-M01]

Western Blot: Factor IX Antibody (2C9) [H00002158-M01] - Analysis of F9 over-expressed 293 cell line, cotransfected with F9 Validated Chimera RNAi ( Cat # H00002158-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F9 monoclonal antibody (M01), clone 2C9 (Cat # H00002158-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunoprecipitation: Factor IX Antibody (2C9) [H00002158-M01]

Immunoprecipitation: Factor IX Antibody (2C9) [H00002158-M01]

Immunoprecipitation: Factor IX Antibody (2C9) [H00002158-M01] - Analysis of F9 transfected lysate using anti-F9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F9 MaxPab rabbit polyclonal antibody.
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Applications for Factor IX Antibody (2C9)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Coagulation Factor IX

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Alternate Names

Christmas Factor, F9, FIX, HEMB, P19, Plasma Thromboplastic Component, PTC, THPH8

Entrez Gene IDs

2158 (Human)

Gene Symbol

F9

OMIM

306900 (Human)

UniProt

NP_000124 (Human)

Additional Coagulation Factor IX Products

Product Documents for Factor IX Antibody (2C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Factor IX Antibody (2C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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