Factor IX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33581

Novus Biologicals, part of Bio-Techne
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NBP1-33581
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (99%), Rhesus Macaque (96%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Coagulation Factor IX Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Factor IX. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (87%), Porcine (87%), Rabbit (82%), Bovine (82%), Feline (83%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Factor IX Antibody

Immunocytochemistry/ Immunofluorescence: Factor IX Antibody [NBP1-33581]

Immunocytochemistry/ Immunofluorescence: Factor IX Antibody [NBP1-33581]

Immunocytochemistry/Immunofluorescence: Factor IX Antibody [NBP1-33581] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX protein stained by Factor IX antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581]

Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581]

Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581] - Mouse liver. Factor IX stained by Factor IX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581]

Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581]

Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581] - Paraffin-embedded human colon cancer. Factor IX antibody [N3C3] diluted at 1:250.
View 2 more images

Applications for Factor IX Antibody

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Coagulation Factor IX

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. Coagulation factor IX is a single chain polypeptide. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Normal concentrations of factor IX in human plasma is app. 3 ug/ml.

Alternate Names

Christmas Factor, F9, FIX, HEMB, P19, Plasma Thromboplastic Component, PTC, THPH8

Gene Symbol

F9

Additional Coagulation Factor IX Products

Product Documents for Factor IX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Factor IX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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