Factor XII heavy chain Antibody (10-11-5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41375

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 10-11-5

Format

BSA Free

Concentration

1 mg/ml

View all Factor XII heavy chain Antibodies »

Product Specifications

Immunogen

Affinity purified human factor XII.

Specificity

Antibody binds the heavy chain of human factor XII, including factor XII derivatives such as a-factor XIIa and a-factor XIIa C1-inhibitor complexes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Factor XII heavy chain Antibody (10-11-5) - BSA Free

Sandwich ELISA: Factor XII heavy chain Antibody (10-11-5) - BSA Free [NBP2-41375] - Analysis using the Biotin conjugate of NBP2-41375. The calibration curveof a sandwich assay using NBP2-41376B as the biotinylated detection antibody and NBP2-41377 as the capture antibody.

Applications for Factor XII heavy chain Antibody (10-11-5) - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-41375 was used in ELISA. Biotinylated NBP2-41375 (1 ug/mL) was used as a detection antibody when used in combination with NBP2-41377 as a capture antibody (1.6 ug/mL) to measure Total alpha-factor XIIa and alpha-factor XIIa C1-inhibitor complexes). (1) WB: NBP2-41375 was used in western blot to detect factor XII, alpha-factor XIIa C1-inh complexes and alpha-factor XIIa.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Factor XII heavy chain

The F12 gene encodes a 615 amino acid long, 67 kDA serum glycoprotein coagulation factor XII that is eventually cleaved into four chains. Factor XII functions in the initiation of blood coagulation, fibrinolysis, as well as in the creation of bradykinin and angiotension. F12 participates in cell adhesion plasmin signaling, blood coagulation signaling pathways, MSP-RON signaling, intrinsic prothrombin activation pathways, and blood clotting cascades. It interacts with Genes CD93, MMP13, EPAS1, APP, and KLKB1. Defects in F12 lead to factor Xii deficiency (Hageman factor deficiency) as well as hereditary angioedema type 3 (estrogen-related HAE). F12 is also associated with blood clots, acute myocardial infarction, thrombosis, spastic paraplegia, angiodema, retinal vein occlusion, arterial calcification, heart disease, C1 inhibitor deficiency, hemorrhagic disease, and acalculous cholecystitis.

Alternate Names

beta-factor XIIa part 1, coagulation factor XII, coagulation factor XII (Hageman factor), coagulation factor XIIa heavy chain, coagulation factor XIIa light chain, EC 3.4.21, EC 3.4.21.38, HAE3, HAEX, HAFbeta-factor XIIa part 2, Hageman factor

Gene Symbol

F12

Additional Factor XII heavy chain Products

Product Documents for Factor XII heavy chain Antibody (10-11-5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Factor XII heavy chain Antibody (10-11-5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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