Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70696

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all F13A1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human F13A1 (NP_000120) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

79.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free

Western Blot: Factor XIIIa Antibody (OTI6F5)Azide and BSA Free [NBP2-70696]

Western Blot: Factor XIIIa Antibody (OTI6F5)Azide and BSA Free [NBP2-70696]

Western Blot: Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free [NBP2-70696] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Factor XIIa.
Immunohistochemistry: Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free [NBP2-70696]

Immunohistochemistry: Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free [NBP2-70696]

Immunohistochemistry: Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free [NBP2-70696] - Analysis of Human lymphoma tissue.
Flow Cytometry: Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free [NBP2-70696]

Flow Cytometry: Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free [NBP2-70696]

Flow Cytometry: Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free [NBP2-70696] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by F13A1 antibody.
View 11 more images

Applications for Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: F13A1

Factor XIII is a beta globulin found in plasma and is composed of two subunits. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Factor XIII A is the catalytic subunit and is a dimmer of molecular weight 160kDa. The B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIII A, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Factor XIII A is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system.

Long Name

Coagulation Factor XIII, A1 Polypeptide

Alternate Names

F13A, FSF, A Subunit

Gene Symbol

F13A1

Additional F13A1 Products

Product Documents for Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Factor XIIIa Antibody (OTI6F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.