Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FADD Antibodies »

Product Specifications

Immunogen

The immunogen is a peptide made to the amino acid region FADD

Specificity

Human FADD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for FADD Antibody (1A11)

Western Blot: FADD Antibody (1A11) [NBP1-28589]

Western Blot: FADD Antibody (1A11) [NBP1-28589]

Western Blot: FADD Antibody (1A11) [NBP1-28589] - Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human FADD-UNLB Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.

Applications for FADD Antibody (1A11)

Application
Recommended Usage

ELISA

1:3000-1:5000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FADD

Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1 beta-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation. (1-6)

Long Name

Fas-associated Death Domain Protein

Alternate Names

MORT1

Entrez Gene IDs

8772 (Human)

Gene Symbol

FADD

UniProt

Q13158

Additional FADD Products

Product Documents for FADD Antibody (1A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FADD Antibody (1A11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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