FADD Antibody (JE32-76)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32327
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE32-76
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide. (Uniprot: Q61160)
Localization
Cytoplasm, cytosol, neuron projection, nucleus, plasma membrane, ripoptosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FADD Antibody (JE32-76)
Western Blot: FADD Antibody (JE32-76) [NBP3-32327]
Western Blot: FADD Antibody (JE32-76) [NBP3-32327] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32327) at 1/1,000 dilution.Lane 1: NIH/3T3 cell lysate (10 ug/Lane)
Lane 2: C6 cell lysate (10 ug/Lane)
Lane 3: Mouse kidney tissue lysate (20 ug/Lane)
Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 30 seconds; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32327) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for FADD Antibody (JE32-76)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FADD
Long Name
Fas-associated Death Domain Protein
Alternate Names
MORT1
Gene Symbol
FADD
Additional FADD Products
Product Documents for FADD Antibody (JE32-76)
Product Specific Notices for FADD Antibody (JE32-76)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...