FANCD2 Antibody (1290D) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80729
Recombinant Monoclonal Antibody
![Knockout Validated: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Azide-and-BSA-Free-Knockout-Validated-NBP2-80729-img0003.jpg "Knockout Validated: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]")
![Western Blot: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Azide-and-BSA-Free-Western-Blot-NBP2-80729-img0001.jpg "Western Blot: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1290D
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This FANCD2 Antibody (1290D) - Azide and BSA Free was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FANCD2 Antibody (1290D) - Azide and BSA Free
Western Blot: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]
Western Blot: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729] - Total protein from HeLa and K562 cells (Molecular weight: 164.1 KDa) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.1 ug/ml FANCD2 Antibody (1290D) (catalog number NBP2-54808) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80729-img0002.jpg "Immunohistochemistry-Paraffin: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]")
Immunohistochemistry-Paraffin: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]
Immunohistochemistry-Paraffin: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Antibody (1290D) (Catalog # NBP2-54808) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM)![Simple Western: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Azide-and-BSA-Free-Simple-Western-NBP2-80729-img0004.jpg "Simple Western: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]")
Simple Western: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]
Simple Western: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729] - Detection of Human FANCD2 by Simple Western using FANCD2 Antibody (1290D) in HeLa and K562 cell lysate. Image from the standard format of this antibody.Applications for FANCD2 Antibody (1290D) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug/ml
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Paraffin
5-10 ug/ml
Simple Western
1 ug/ml
Western Blot
0.1 - 0.5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FANCD2
References
1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K., . . . Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014
2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B., . . . Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1
Long Name
Fanconi Anemia, Complementation Group D2
Alternate Names
FA4, FACD, FAD, FAD2, FANCD
Gene Symbol
FANCD2
Additional FANCD2 Products
Product Documents for FANCD2 Antibody (1290D) - Azide and BSA Free
Product Specific Notices for FANCD2 Antibody (1290D) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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