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FARS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85187

Novus Biologicals, part of Bio-Techne
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NBP1-85187
NBP1-85187-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKYPAVINDISFWLPSENYAEND

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FARS2 Antibody

Western Blot: FARS2 Antibody [NBP1-85187]

Western Blot: FARS2 Antibody [NBP1-85187]

Western Blot: FARS2 Antibody [NBP1-85187] - Analysis in control (vector only transfected HEK293T lysate) and FARS2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: FARS2 Antibody [NBP1-85187]

Immunocytochemistry/ Immunofluorescence: FARS2 Antibody [NBP1-85187]

Immunocytochemistry/Immunofluorescence: FARS2 Antibody [NBP1-85187] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Immunohistochemistry-Paraffin: FARS2 Antibody [NBP1-85187]

Immunohistochemistry-Paraffin: FARS2 Antibody [NBP1-85187]

Immunohistochemistry-Paraffin: FARS2 Antibody [NBP1-85187] - Staining of human kidney shows strong cytoplasmic and extra cellular positivity in cells in tubules.

Applications for FARS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FARS2

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. [provided by RefSeq]

Alternate Names

dJ236A3.1, dJ520B18.2, dJ520B18.2 (FARS1 (phenylalanine-tRNA synthetase)), EC 6.1.1, EC 6.1.1.20, FARS1dJ236A3.1 (phenylalanine-tRNA synthetase), HSPC320, phenylalanine translase, phenylalanine tRNA ligase 2, mitochondrial, Phenylalanine--tRNA ligase, phenylalanine-tRNA synthetase 1 (mitochondrial), phenylalanine-tRNA synthetase 2 (mitochondrial), phenylalanyl-tRNA synthetase 2, mitochondrial, phenylalanyl-tRNA synthetase, mitochondrial, PheRS

Gene Symbol

FARS2

Additional FARS2 Products

Product Documents for FARS2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FARS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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