Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09151

Clone NOK-1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP3-09151
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NOK-1

Format

BSA Free

Concentration

1 mg/ml

View all Fas Ligand/TNFSF6 Antibodies »

Product Summary for Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free

Immunogen

L5178Y mouse T lymphoma cells expressing recombinant human Fas Ligand/TNFSF6

Specificity

The mouse monoclonal antibody NOK-1 recognizes an extracellular epitope of CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free [NBP3-09151]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free [NBP3-09151]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) [NBP3-09151] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free [NBP3-09151]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free [NBP3-09151]

Flow Cytometry: Fas Ligand/TNFSF6 Antibody (NOK-1) [NBP3-09151] - Surface staining pattern of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml) GAM APC.

Applications for Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fas Ligand/TNFSF6

Fas / APO-1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand (Fas-L) or an anti-Fas cross-linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas-L system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand (Fas-L), which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas-L system. Fas is believed to be involved in various autoimmune diseases including, ulcerative colitis, Graves disease, and rheumatoid arthritis. Fas expression on gastric epithelial cells in patients infected with H.Pylori is responsible for the accelerated apoptosis of the cells. Serum Fas-L concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension.

Alternate Names

CD178, CD95L, FASLG, TNFSF6

Gene Symbol

FASLG

Additional Fas Ligand/TNFSF6 Products

Product Documents for Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fas Ligand/TNFSF6 Antibody (NOK-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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