Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-503

Novus Biologicals, part of Bio-Techne
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NB500-503
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LT95

Format

BSA Free

Concentration

1 mg/ml

View all Fas/TNFRSF6/CD95 Antibodies »

Product Specifications

Immunogen

HUT-78 human T cell lymphoma cell line

Localization

Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).

Specificity

The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free

Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free [NB500-503]

Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free [NB500-503]

Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - ytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody (DX2) Green - anti-CD95 (LT95) A - JURKAT human peripheral blood T cell leukemia cell line B - RAMOS human Burkitt lymphoma cell line C - CEM human leukemia cell line D - MOLT-4 human acute lymphoblastic T cell leukemia cell line
Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free [NB500-503]

Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free [NB500-503]

Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fig. 1. Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining Black - standard anti-CD95 monoclonal antibody Green - anti-CD95 (LT95) A. JURKAT human peripheral blood T cell leukemia cell line B. RAMOS human Burkitt lymphoma cell line C. CEM human leukemia cell line D. MOLT-4 human acute lymphoblastic T cell leukemia cell line.
Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free [NB500-503]

Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free [NB500-503]

Flow Cytometry: Fas Receptor/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - Fas/TNFRSF6/CD95 Antibody (LT95) [NB500-503] - A surface stain was performed on HeLa with Fas/TNFRSF6/CD95 Antibody (LT95) NB500-503AF647 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Applications for Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-12 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Fas/TNFRSF6/CD95

Tumor Necrosis Family Receptor (TNFR) superfamily member Fas, also known as CD95, APO-1, and TNFRSF6, is a 40-50 kDa type I transmembrane glycoprotein that is traditionally considered a death receptor but also functions in non-apoptotic signaling (1-4). The human Fas/TNFRSF6/CD95 protein is encoded by the FAS gene which contains 9 exons and is located on chromosome 10 (10q23.3-4) (1,2). The mature canonical Fas/TNFRSF6 protein isoform is 335 aa in length, which includes the signal sequence, and has a theoretical molecular weight of 37.7 kDa (1,5). The protein contains an extracellular domain (ECD) consisting of three calcium rich domains (CRDs), a transmembrane domain (TM), and an intracellular domain (ICD) comprised of a calcium-inducing domain (CID) and characteristic dead domain (DD) (1,2,5,6). The Fas protein is expressed on the plasma membrane of activated lymphocytes as a homotrimer formed via CRD1 interactions (1,2,3,6). The DD is crucial for apoptotic signaling which is triggered by the Fas receptor binding its ligand, Fas ligand (FasL) (1,2,6,7). Upon Fas-FasL interaction, the DD recruits an adapter protein Fas-associated DD (FADD) and procaspase-8, generating the death-inducing signaling complex (DISC) (1-4,6-8). Formation of DISC activates caspase-8 and leads to cleavage of caspase-3, initiating a caspase-signaling cascade and cell death (1-4,6-8).

Fas-FasL-mediated apoptosis is important in immune homeostasis and removal of autoreactive T cells, autoreactive B cells, cytotoxic natural killer (NK) cells, and more (1,2,7). Dysfunction and mutations in the Fas receptor and the Fas-FasL signaling axis is associated a loss of apoptotic signaling and removal of autoreactive cells, which correlates with several autoimmune diseases including systemic lupus erythematosus (SLE), autoimmune lymphoproliferative syndrome (ALPS), and multiple sclerosis (MS) (1-4,6,7). In addition to apoptosis and cell death signaling, FasL/TNFRSF6/CD95 mediates other pathways involved in proliferation, survival, and differentiation (3,4,6,8). More specifically, Fas has been shown to activate the NF-kappaB pathway, driving innate immunity which includes IL-1beta production and functioning in host defense (3,4,6,8). Fas is also involved in adaptive immunity playing a role in co-stimulation of CD4+ and CD8+ T cell activation as well as precocious differentiation of naive cells to effector memory T cells (3,4,6). Differentiation into effector memory T cells shows protection against autoimmunity but also limits antitumor response to a form of cancer immunotherapy called adoptive cell transfer (ACT) (3,4). The non-apoptotic roles of the Fas/TNFRSF6/CD95 receptor highlight its potential as a target for both treating autoimmune diseases and in cancer immunotherapy (3,4).

References

1. Singh R, Pradhan V, Patwardhan M, Ghosh K. APO-1/Fas gene: Structural and functional characteristics in systemic lupus erythematosus and other autoimmune diseases. Indian J Hum Genet. 2009;15(3):98-102. https://doi.org/10.4103/0971-6866.60184

2. Magerus A, Bercher-Brayer C, Rieux-Laucat F. The genetic landscape of the FAS pathway deficiencies. Biomed J. 2021;44(4):388-399. https://doi.org/1010.1016/j.bj.2021.06.005

3. Guegan JP, Legembre P. Nonapoptotic functions of Fas/CD95 in the immune response. FEBS J. 2018;285(5):809-827. https://doi.org/10.1111/febs.14292

4. Yi F, Frazzette N, Cruz AC, Klebanoff CA, Siegel RM. Beyond Cell Death: New Functions for TNF Family Cytokines in Autoimmunity and Tumor Immunotherapy. Trends Mol Med. 2018;24(7):642-653. https://doi.org/10.1016/j.molmed.2018.05.004

5. Uniprot (P25445)

6. Guegan JP, Ginestier C, Charafe-Jauffret E, et al. CD95/Fas and metastatic disease: What does not kill you makes you stronger. Semin Cancer Biol. 2020;60:121-131. https://doi.org/10.1016/j.semcancer.2019.06.004

7. Volpe E, Sambucci M, Battistini L, Borsellino G. Fas-Fas Ligand: Checkpoint of T Cell Functions in Multiple Sclerosis. Front Immunol. 2016;7:382. Published 2016 Sep 27. https://doi.org/10.3389/fimmu.2016.00382

8. Cullen SP, Martin SJ. Fas and TRAIL 'death receptors' as initiators of inflammation: Implications for cancer. Semin Cell Dev Biol. 2015;39:26-34. https://doi.org/10.1016/j.semcdb.2015.01.012

Long Name

Fibroblast-associated

Alternate Names

Apo-1, APT1, CD95, TNFRSF6

Gene Symbol

FAS

Additional Fas/TNFRSF6/CD95 Products

Product Documents for Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free

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Product Specific Notices for Fas/TNFRSF6/CD95 Antibody (LT95) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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