FAT2 Antibody (8C5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53235
![Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235]](https://resources.bio-techne.com/images/products/FAT2-Antibody-8C5-Immunohistochemistry-Paraffin-NBP2-53235-img0002.jpg "Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8C5
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Purified recombinant human FAT2 protein. (Uniprot: Q9NYQ8)
Localization
Cell Surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
480 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54371)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for FAT2 Antibody (8C5)
Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235]
Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).![Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235]](https://resources.bio-techne.com/images/products/FAT2-Antibody-8C5-Immunohistochemistry-Paraffin-NBP2-53235-img0001.jpg "Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235]")
Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235]
Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).Applications for FAT2 Antibody (8C5)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: FAT2
Alternate Names
CDHF8, CDHR9, FAT tumor suppressor homolog 2 (Drosophila), HFAT2, MEGF1, protocadherin Fat 2
Gene Symbol
FAT2
UniProt
Additional FAT2 Products
Product Documents for FAT2 Antibody (8C5)
Product Specific Notices for FAT2 Antibody (8C5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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