FAT2 Antibody (8C5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54371

Novus Biologicals, part of Bio-Techne
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NBP2-54371-100ug
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8C5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all FAT2 Antibodies »

Product Specifications

Immunogen

Purified recombinant human FAT2 protein. (Uniprot: Q9NYQ8)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

480 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53235).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for FAT2 Antibody (8C5) - Azide and BSA Free

Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) - Azide and BSA Free [NBP2-54371]

Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) - Azide and BSA Free [NBP2-54371]

Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-54371] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).
Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) - Azide and BSA Free [NBP2-54371]

Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) - Azide and BSA Free [NBP2-54371]

Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-54371] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).

Applications for FAT2 Antibody (8C5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: FAT2

The FAT2 gene is the second identified human homolog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of

Alternate Names

CDHF8, CDHR9, FAT tumor suppressor homolog 2 (Drosophila), HFAT2, MEGF1, protocadherin Fat 2

Gene Symbol

FAT2

Additional FAT2 Products

Product Documents for FAT2 Antibody (8C5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FAT2 Antibody (8C5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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