FAT4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78381

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FAT4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7]

Localization

Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAT4 Antibody - BSA Free

Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381]

Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381]

Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381] - FAT4 Antibody [NBP1-78381] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.
Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381]

Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381]

Knockout Validated: FAT4 Antibody [NBP1-78381] - IHC staining of metastatic lung nodules in the nude mice model. Representative images show IHC staining with anti-FAT4 antibody of lung sections from BGC-823-FAT4-KO and BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 i1/4m. Image collected and cropped by Citeab from the following publication (Low FAT4 expression is associated with a poor prognosis in gastric cancer patients. Oncotarget (2018) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: FAT4 Antibody - BSA Free [NBP1-78381]

Immunocytochemistry/ Immunofluorescence: FAT4 Antibody - BSA Free [NBP1-78381]

Immunocytochemistry/Immunofluorescence: FAT4 Antibody [NBP1-78381] - Antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
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Applications for FAT4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

reported in scientific literature (PMID 29321622)
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAT4

FAT4 (FAT tumor suppressor homolog 4, also know as Fat-J, mFat4, and CDHF14) is a member of the Cadherin superfamily. Members of the Cadherin superfamily positively regulate planar cell polarity (PCP) in drosophila wings and play an important role in cell to cell adhesion interactions. FAT4 and other Cadherin family members have been used in systems medicine; especially in the fields of oncology and neurology (PMID: 16865240). FAT4 is widely expressed in most tissues, especially fetal and infant brain. It is also expressed in brain tumors and colorectal cancer. Furthermore, human breast tumor cell lines and primary tumors show a loss of FAT4 expression. Promoter methylation of a FAT4 allele can inactivate the expression of the FAT4 gene in deleted regions of the Hippo signaling pathway, the pathway that controls organ size (PMID: 19048595).

Alternate Names

cadherin family member 14, cadherin-related family member 11, CDHR11, FAT tumor suppressor homolog 4 (Drosophila), FAT-J, fat-like cadherin protein FAT-J, FLJ77878, hFat4, NBLA00548, putative protein product of Nbla00548

Entrez Gene IDs

79633 (Human)

Gene Symbol

FAT4

UniProt

Q6V0I7 (Human)

Additional FAT4 Products

Product Documents for FAT4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FAT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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