FAT4 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78381F

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FAT4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7]

Localization

Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAT4 Antibody [FITC]

Knockout Validated: FAT4 Antibody [FITC] [NBP1-78381F] - Representative images show IHC staining with anti-FAT4 antibody (NBP1-78381F) of lung sections from BGC-823-FAT4-KO and BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.
Knockout Validated: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.
FAT4 Antibody [FITC]

Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -

Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - H&E & IHC staining of metastatic lung nodules in the nude mice model(A) Representative images (40X, 100X & 400X) of H&E stained lung sections from nude mice injected via the tail vein with BGC-823-FAT4-KO & BGC-823-KO-NC cells. (B) Representative images show IHC staining with anti-FAT4 antibody of lung sections from BGC-823-FAT4-KO & BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 μm. (C) Histogram shows Ki-67 expression & index (%) in lungs of the BGC-823-FAT4-KO & BGC-823-KO-NC group mice. As shown, the BGC-823-FAT4-KO group shows higher Ki-67 index (%) than BGC-823-KO-NC group. Data represent mean ± SD from 3 independent experiments. Scale bar, 100 μm. * denotes P < 0.05 compared to control. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
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Applications for FAT4 Antibody [FITC]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FAT4

FAT4 (FAT tumor suppressor homolog 4, also know as Fat-J, mFat4, and CDHF14) is a member of the Cadherin superfamily. Members of the Cadherin superfamily positively regulate planar cell polarity (PCP) in drosophila wings and play an important role in cell to cell adhesion interactions. FAT4 and other Cadherin family members have been used in systems medicine; especially in the fields of oncology and neurology (PMID: 16865240). FAT4 is widely expressed in most tissues, especially fetal and infant brain. It is also expressed in brain tumors and colorectal cancer. Furthermore, human breast tumor cell lines and primary tumors show a loss of FAT4 expression. Promoter methylation of a FAT4 allele can inactivate the expression of the FAT4 gene in deleted regions of the Hippo signaling pathway, the pathway that controls organ size (PMID: 19048595).

Alternate Names

cadherin family member 14, cadherin-related family member 11, CDHR11, FAT tumor suppressor homolog 4 (Drosophila), FAT-J, fat-like cadherin protein FAT-J, FLJ77878, hFat4, NBLA00548, putative protein product of Nbla00548

Gene Symbol

FAT4

Additional FAT4 Products

Product Documents for FAT4 Antibody [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FAT4 Antibody [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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