FAT4 Antibody [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78381F
![Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -](https://resources.bio-techne.com/images/products/FAT4-Antibody-[FITC]-Knockout-Validated-NBP1-78381F-img0001.jpg)
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7]
Localization
Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAT4 Antibody [FITC]
Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -
Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - Representative images show IHC staining with anti-FAT4 antibody (NBP1-78381F) of lung sections from BGC-823-FAT4-KO and BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -
Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -
Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model(A) Nude mice xenografted (n=5) with BGC-823-FAT4-KO cells (left side) show increased tumor growth (size) than tumors in mice xenografted with control BGC-803-NC cells (right side). (B) BGC-823-FAT4-KO cell derived xenograft tumors show increased tumor volume than control BGC-823-KO-NC cells (*P<0.05). The data points represent mean tumor volume ± SD measured on the indicated days. (C) Representative images show IHC staining of BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors with antibodies against FAT4 & Ki-67. Scale bar, 100 μm. (D) Histogram plots show percent Ki-67 positive cells in BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors. As shown, the Ki-67 index is higher in the BGC-823-FAT4-KO derived tumors than BGC-823-KO-NC derived tumors. Data represent mean ± SD from 3 independent experiments. * denotes P < 0.05 compared to control. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for FAT4 Antibody [FITC]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FAT4
Alternate Names
cadherin family member 14, cadherin-related family member 11, CDHR11, FAT tumor suppressor homolog 4 (Drosophila), FAT-J, fat-like cadherin protein FAT-J, FLJ77878, hFat4, NBLA00548, putative protein product of Nbla00548
Gene Symbol
FAT4
Additional FAT4 Products
Product Documents for FAT4 Antibody [FITC]
Product Specific Notices for FAT4 Antibody [FITC]
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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