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Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25462

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25462

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2160

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Immunogen

Recombinant fragment of human Fatty Acid Synthase/FASN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] -

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Fatty Acid Synthase antibody [HL2160] detects Fatty Acid Synthase protein at lipid droplet by immunofluorescent analysis. Sample: Mock and differentiated 3T3-L1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:500. Blue: Fluoroshield with DAPI .
Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] -

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Fatty Acid Synthase antibody [HL2160] detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat breast. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Western Blot: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] -

Western Blot: Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free [NBP3-25462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2160] (NBP3-25462) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fatty Acid Synthase/FASN

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Alternate Names

FASN, OA-519, SDR27X1

Gene Symbol

FASN

Additional Fatty Acid Synthase/FASN Products

Product Documents for Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fatty Acid Synthase/FASN Antibody (HL2160) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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