Fatty Acid Synthase/FASN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84733

Novus Biologicals, part of Bio-Techne
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NBP1-84733
NBP1-84733-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Fatty Acid Synthase/FASN Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RWLRVTVAGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSERAALQEELQLCKGLVQALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRLLSAACRLQLNGN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fatty Acid Synthase/FASN Antibody

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq data are presented for the same tissues.
Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733]

Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733]

Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Analysis using Anti-FASN antibody NBP1-84733 (A) shows similar pattern to independent antibody NBP2-49213 (B).
Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733]

Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733]

Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Analysis in human cell line SK-BR-3.
View 3 more images

Applications for Fatty Acid Synthase/FASN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 22951905). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fatty Acid Synthase/FASN

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Alternate Names

FASN, OA-519, SDR27X1

Gene Symbol

FASN

Additional Fatty Acid Synthase/FASN Products

Product Documents for Fatty Acid Synthase/FASN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fatty Acid Synthase/FASN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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