FBXW7/Cdc4 Antibody (3D1)

Novus Biologicals, part of Bio-Techne | Catalog # H00055294-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FBXW7/Cdc4 Antibodies »

Product Specifications

Immunogen

FBXW7 (NP_361014, 599 a.a. ~ 707 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK

Reactivity Notes

Human. Other species not tested.

Specificity

FBXW7 - F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for FBXW7/Cdc4 Antibody (3D1)

Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody (3D1) [H00055294-M02]

Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody (3D1) [H00055294-M02]

Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody (3D1) [H00055294-M02] - Analysis of monoclonal antibody to FBXW7 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
Sandwich ELISA: FBXW7/Cdc4 Antibody (3D1) [H00055294-M02] - Detection limit for recombinant GST tagged FBXW7 is approximately 0.1ng/ml as a capture antibody.

Applications for FBXW7/Cdc4 Antibody (3D1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FBXW7/Cdc4

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.

Long Name

F-box and WD Repeat Domain Containing 7

Alternate Names

AGO, Archipelago Homolog, Cdc4, FBW6, Fbw7, FBX30, FBXO30, FBXW6, SCF(Fbw7), SEL10

Entrez Gene IDs

55294 (Human)

Gene Symbol

FBXW7

OMIM

606278 (Human)

UniProt

NP_361014 (Human)

Additional FBXW7/Cdc4 Products

Product Documents for FBXW7/Cdc4 Antibody (3D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FBXW7/Cdc4 Antibody (3D1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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