Fc epsilon RI Antibody (2C12-3B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00002205-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C12-3B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Fc epsilon RI Antibodies »

Product Specifications

Immunogen

FCER1A (AAH05912.1, 1 a.a. ~ 257 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPLLVVILFAVDTGLFISTQQQVTFLLKIKRTRKGFRLLNPHPKPNPKNN

Specificity

FCER1A - Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Fc epsilon RI Antibody (2C12-3B6)

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01]

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01]

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.Lane 1: FCER1A transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.
Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01]

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01]

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - Analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01]

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01]

Western Blot: Fc epsilon RI Antibody (2C12-3B6) [H00002205-M01] - FCER1A monoclonal antibody (M01), clone 2C12-3B6 Analysis of FCER1A expression in A-549.
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Applications for Fc epsilon RI Antibody (2C12-3B6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and RNAi Validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fc epsilon RI

The IgE receptor plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more high-affinity IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. The IgE receptor consists of 3 subunits: alpha, beta (MIM 147138), and gamma (MIM 147139); only the alpha subunit is glycosylated.[supplied by OMIM]

Alternate Names

alpha polypeptide, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, high-affinity, of mast cells, alpha polypeptide

Entrez Gene IDs

2205 (Human)

Gene Symbol

FCER1A

OMIM

147140 (Human)

UniProt

AAH05912 (Human)

Additional Fc epsilon RI Products

Product Documents for Fc epsilon RI Antibody (2C12-3B6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fc epsilon RI Antibody (2C12-3B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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