Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12050
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free [NBP3-12050]](https://resources.bio-techne.com/images/products/Fc-epsilon-RI-Antibody-IB10-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12050-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free [NBP3-12050]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # IB10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human protein, N-terminus.
Specificity
This antibody binds to human Fc epsilon receptor and competes with IgE binding.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free [NBP3-12050]
Immunocytochemistry/Immunofluorescence: Fc epsilon RI Antibody (IB10) - Chimeric [NBP3-12050] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of IB10 (NBP3-12050) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing diffuse membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12050, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.Applications for Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
IB10 competes with IgE for binding to human FCER1A; consequently, its binding to mast cells and basophils is decreased when IgE concentration is high, but it does block IgE binding to these cells. IB10 and IE7 do not crossblock each other's binding. This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Fc epsilon RI
Alternate Names
alpha polypeptide, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, high-affinity, of mast cells, alpha polypeptide
Gene Symbol
FCER1A
Additional Fc epsilon RI Products
Product Documents for Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free
Product Specific Notices for Fc epsilon RI Antibody (IB10) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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