Fc gamma RI/CD64 Antibody (37F7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42685

Novus Biologicals, part of Bio-Techne
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NBP2-42685
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 37F7

Format

BSA Free

Concentration

1 mg/ml

View all Fc gamma RI/CD64 Antibodies »

Product Summary for Fc gamma RI/CD64 Antibody (37F7) - BSA Free

Immunogen

Recombinant human Fc gamma RI/CD64 (16-292aa) purified from E.coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Fc gamma RI/CD64 Antibody (37F7) - BSA Free

Western Blot: Fc gamma RI/CD64 Antibody (37F7)BSA Free [NBP2-42685]

Western Blot: Fc gamma RI/CD64 Antibody (37F7)BSA Free [NBP2-42685]

Western Blot: Fc gamma RI/CD64 Antibody (37F7) [NBP2-42685] - The recombinant protein (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FCGR1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (37F7) - BSA Free [NBP2-42685]

Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (37F7) - BSA Free [NBP2-42685]

Immunocytochemistry/Immunofluorescence: Fc gamma RI/CD64 Antibody (37F7) [NBP2-42685] - Analysis of FCGR1A in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FCGR1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Fc gamma RI/CD64 Antibody (37F7) - BSA Free [NBP2-42685]

Flow Cytometry: Fc gamma RI/CD64 Antibody (37F7) - BSA Free [NBP2-42685]

Flow Cytometry: Fc gamma RI/CD64 Antibody (37F7) [NBP2-42685] - Analysis of FCGR1A in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Applications for Fc gamma RI/CD64 Antibody (37F7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fc gamma RI/CD64

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Long Name

Fc gamma Receptor I

Alternate Names

CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA

Gene Symbol

FCGR1A

UniProt

NP_000557

Additional Fc gamma RI/CD64 Products

Product Documents for Fc gamma RI/CD64 Antibody (37F7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fc gamma RI/CD64 Antibody (37F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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