Fc gamma RI/CD64 Antibody (H22) [DyLight 594]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81098DL594
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Competitive ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
DyLight 594 (Excitation = 593 nm, Emission = 618 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # H22
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Fc gamma RI/CD64 Antibody (H22) [DyLight 594]
Immunogen
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.
Specificity
This immunotoxin is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Applications for Fc gamma RI/CD64 Antibody (H22) [DyLight 594]
Application
Recommended Usage
Competitive ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fc gamma RI/CD64
Long Name
Fc gamma Receptor I
Alternate Names
CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA
Gene Symbol
FCGR1A
Additional Fc gamma RI/CD64 Products
Product Documents for Fc gamma RI/CD64 Antibody (H22) [DyLight 594]
Product Specific Notices for Fc gamma RI/CD64 Antibody (H22) [DyLight 594]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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