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Fc gamma RII/CD32 Antibody (AT10) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63714

Novus Biologicals, part of Bio-Techne
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NB100-63714
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # AT10

Concentration

0.1 mg/ml

Product Specifications

Immunogen

K562 cell line

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33114132).

Specificity

NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fc gamma RII/CD32 Antibody (AT10) [FITC]

Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [FITC] [NB100-63714] - Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD32:FITC.

Applications for Fc gamma RII/CD32 Antibody (AT10) [FITC]

Application
Recommended Usage

Flow Cytometry

Neat-1:10
Application Notes
This antibody does not work in Immunohistochemistry-Paraffin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fc gamma RII/CD32

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Gene Symbol

FCGR2B

Additional Fc gamma RII/CD32 Products

Product Documents for Fc gamma RII/CD32 Antibody (AT10) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fc gamma RII/CD32 Antibody (AT10) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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