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Fc gamma RII/CD32 Antibody (AT10) [mFluor Violet 450 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65338MFV450

Novus Biologicals, part of Bio-Techne
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NB100-65338MFV450
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # AT10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

K562 cell line

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33114132).

Specificity

NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fc gamma RII/CD32 Antibody (AT10) [mFluor Violet 450 SE]

Fc gamma RII/CD32 Antibody (AT10) [mFluor Violet 450 SE] [NB100-65338MFV450] -

Fc gamma RII/CD32 Antibody (AT10) [mFluor Violet 450 SE] [NB100-65338MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

Applications for Fc gamma RII/CD32 Antibody (AT10) [mFluor Violet 450 SE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fc gamma RII/CD32

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Gene Symbol

FCGR2B

Additional Fc gamma RII/CD32 Products

Product Documents for Fc gamma RII/CD32 Antibody (AT10) [mFluor Violet 450 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fc gamma RII/CD32 Antibody (AT10) [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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