Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 190909

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fc gamma RII/RIII (CD32/CD16) Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr30-Arg207
Accession # P08101

Specificity

Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse CD16 is observed and no cross-reactivity with recombinant human CD32 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (190909) [PE/Cy5.5]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Fc gamma RII/RIII (CD32/CD16)

Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. In humans each group of receptors is encoded by 2 - 3 closely related genes designated A, B, and C. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Different Fc receptors can function as activators, as inhibitors, or as decoy receptors.

Long Name

Fc gamma Receptor II/III

Alternate Names

CD32/CD16

Additional Fc gamma RII/RIII (CD32/CD16) Products

Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (190909) [PE/Cy5.5]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (190909) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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