Fc gamma RII/RIII (CD32/CD16) Antibody (J5511)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30971

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # J5511

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fc gamma RII/RIII (CD32/CD16) Antibodies »

Product Specifications

Immunogen

CD16=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with NK lymphocytes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (J5511)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
PBMC: Add10 ul of MAB/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacture's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Phosphate buffered saline

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fc gamma RII/RIII (CD32/CD16)

Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. In humans each group of receptors is encoded by 2 - 3 closely related genes designated A, B, and C. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Different Fc receptors can function as activators, as inhibitors, or as decoy receptors.

Long Name

Fc gamma Receptor II/III

Alternate Names

CD32/CD16

Gene Symbol

FCGR3A

Additional Fc gamma RII/RIII (CD32/CD16) Products

Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (J5511)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (J5511)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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