Fc gamma RIIB/CD32b Antibody (2E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00002213-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Fc gamma RIIB/CD32b Antibodies »

Product Specifications

Immunogen

FCGR2B (AAH31992, 45 a.a. ~ 154 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AAPPKAVLKLEPQWINVLQEDSVTLTCRGTHSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIVVRCHS

Specificity

FCGR2B - Fc fragment of IgG, low affinity IIb, receptor (CD32)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Fc gamma RIIB/CD32b Antibody (2E10)

Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01]

Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01]

Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - Analysis of FCGR2B expression in transfected 293T cell line by FCGR2B monoclonal antibody (M01), clone 2E10.Lane 1: FCGR2B transfected lysate(34 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01]

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01]

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - Analysis of monoclonal antibody to FCGR2B on formalin-fixed paraffin-embedded human placenta. Antibody concentration 6 ug/ml.
Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01]

Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01]

Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - FCGR2B monoclonal antibody (M01), clone 2E10 Analysis of FCGR2B expression in K-562.
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Applications for Fc gamma RIIB/CD32b Antibody (2E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fc gamma RIIB/CD32b

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Long Name

Fc gamma Receptor II

Alternate Names

CD32b, FCGR2B, FcgRIIB, FCRIIB

Entrez Gene IDs

2213 (Human)

Gene Symbol

FCGR2B

OMIM

604590 (Human)

UniProt

AAH31992 (Human)

Additional Fc gamma RIIB/CD32b Products

Product Documents for Fc gamma RIIB/CD32b Antibody (2E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fc gamma RIIB/CD32b Antibody (2E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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