Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37713

Novus Biologicals, part of Bio-Techne
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NBP2-37713
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BSA Free
NB500-378
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Block/Neutralize, CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Functional, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-154

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Fc gamma RIII (CD16) Antibodies »

Product Summary for Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free

Immunogen

Human granulocytes

Specificity

The antibody MEM-154 reacts with an epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes. HLDA V; WS Code M MA068 HLDA V; WS Code NK NK51

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free

Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free [NBP2-37713]

Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free [NBP2-37713]

Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - Azide Free [NBP2-37713] - Staining of human peripheral blood cells with anti-human CD16 (MEM-154) purified, GAM/APC.
Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free [NBP2-37713]

Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free [NBP2-37713]

Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - Azide Free [NBP2-37713] - Surface staining pattern of human peripheral blood stained using anti-human CD16 (MEM-154) purified antibody (low endotoxin, concentration in sample 2 ug/ml) GAM APC.

Applications for Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1:10-1:1000

Flow Cytometry

5-10 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000Blocking/Neutralizing 50-200 molar excess
Application Notes
This antibody does not react with CD16a present on NK cells in many subjects.
Western Blot: non-reducing conditions.
Blocking/Neutralizing: this antibody blocks binding of human IgG to FcgammaRIII.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Formulation

PBS (pH 7.4), 0.2 um filter sterilized

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Fc gamma RIII (CD16)

Fc gamma receptor III (RIII), also called CD16, is an activating natural killer (NK) cell receptor that binds the Fc portion of immunoglobulin G (IgG) antibodies and is responsible for eliciting a host defense against microbial pathogens (1). Fc gamma RIII (CD16) is one of three subclasses of Fc gamma receptors which includes Fc gamma RI (CD64) and Fc gamma RII (CD32) (1,2). The two forms of Fc gamma RIII are Fc gamma RIIIA (CD16a) and Fc gamma RIIIB (CD16b), which are encoded by two different homologous genes, FCGR3A and FCGR3B, respectively (1-3). The human Fc gamma RIIIA protein is 254 amino acids (aa) in length with a theoretical molecular weight (MW) of 29 kDa, while human Fc gamma RIIIB protein is 233 aa long with a calculated MW of 26 kDa (1,4,5). Each protein contains between five to six glycosylation sites (1,4,5).

Fc gamma RIIIA/CD16a is expressed as a transmembrane protein on NK cells and on a subset of monocytes, macrophages, CD4+T cells, basophils, and mast cells (1-3). Fc gamma RIIIB/CD16b is primarily expressed on neutrophils as a glycosylphosphatidylinositol (GPI)-anchored protein but is also expressed on a subset of basophils and can be induced on eosinophils (1-3). The soluble form of CD16 (sCD16) which is often produced following exposure to inflammatory signals and protein shedding via metalloproteinases (3). Reduced sCD16 levels have been found in patients with multiple myeloma (3).

Activating NK cell receptor function has been harnessed for its potential in tumor immunotherapy (6). One immunotherapy strategy is using bi- and tri-specific NK cell engagers (BiKE and TriKE) to target the Fc gamma RIIIA/CD16a receptor with tumor-associated antigens to stimulate a cytotoxic response and mount an attack on tumor cells (6). CD16a is also capable of antibody dependent cellular cytotoxicity (ADCC) through recognition of antibodies bound to target cells (6-7). CD16-induced NK cell activation allows for NK co-receptor expression including stimulatory receptors like CD137 or inhibitory receptors like TIGIT and PD-1, which serve as additional regulatory checkpoints during ADCC (7). Therapeutic antibodies for cancer treatment like rituximab or trastuzumab can be recognized by Fc gamma RIIIA/CD16a to activate NK cell-mediated killing of tumor cells (6-7).

References

1. Fossati, G., Bucknall, R. C., & Edwards, S. W. (2001). Fcgamma receptors in autoimmune diseases. European Journal of Clinical Investigation, 31(9), 821-831. https://doi.org/10.1046/j.1365-2362.2001.00881.x

2. Patel, K. R., Roberts, J. T., & Barb, A. W. (2019). Multiple Variables at the Leukocyte Cell Surface Impact Fc gamma Receptor-Dependent Mechanisms. Frontiers in Immunology, 10, 223. https://doi.org/10.3389/fimmu.2019.00223

3. Moldovan, I., Galon, J., Maridonneau-Parini, I., Roman Roman, S., Mathiot, C., Fridman, W. H., & Sautes-Fridman, C. (1999). Regulation of production of soluble Fc gamma receptors type III in normal and pathological conditions. Immunology Letters, 68(1), 125-134. https://doi.org/10.1016/s0165-2478(99)00041-3

4. Uniprot (P08637)

5. Uniprot (O75015)

6. Sivori, S., Pende, D., Quatrini, L., Pietra, G., Della Chiesa, M., Vacca, P., Tumino, N., Moretta, F., Mingari, M. C., Locatelli, F., & Moretta, L. (2021). NK cells and ILCs in tumor immunotherapy. Molecular Aspects of Medicine, 80, 100870. https://doi.org/10.1016/j.mam.2020.100870

7. Muntasell, A., Ochoa, M. C., Cordeiro, L., Berraondo, P., Lopez-Diaz de Cerio, A., Cabo, M., Lopez-Botet, M., & Melero, I. (2017). Targeting NK-cell checkpoints for cancer immunotherapy. Current Opinion in Immunology, 45, 73-81. https://doi.org/10.1016/j.coi.2017.01.003

Long Name

Fc gamma Receptor III

Alternate Names

FcgRIII

Gene Symbol

FCGR3A

Additional Fc gamma RIII (CD16) Products

Product Documents for Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free

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Product Specific Notices for Fc gamma RIII (CD16) Antibody (MEM-154) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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