Feline CD8 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2598

R&D Systems, part of Bio-Techne
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AF2598
AF2598-SP
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Key Product Details

Species Reactivity

Feline

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CD8 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant feline CD8 alpha
Ala22-Tyr188
Accession # P41688

Specificity

Detects feline CD8 alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CD8 alpha is observed, approximately 5% cross-reactivity with recombinant canine CD8 alpha is observed, and less than 1% cross-reactivity with recombinant human CD8 alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Feline CD8 alpha Antibody

CD8a antibody in Feline PBMCs by Immunocytochemistry (ICC).

CD8 alpha in Feline PBMCs.

CD8a was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline CD8a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Feline CD8 alpha Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed feline peripheral blood mononuclear cells

Western Blot

0.1 µg/mL
Sample: Recombinant Feline CD8 alpha

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members. The CD8 alpha chain is a 34 kDa type I transmembrane glycoprotein that is disulfide-linked, either to itself forming CD8 alpha alpha, or to an unrelated 30-35 kDa CD8 beta chain, forming CD8 alpha beta. CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR coreceptor, while CD8 alpha alpha promotes T cell survival and differentation. Feline CD8 alpha extracellular region shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha extracellular regions, respectively.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P41688

Additional CD8 Products

Product Documents for Feline CD8 alpha Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Feline CD8 alpha Antibody

For research use only

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