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Feline Fas/TNFRSF6/CD95 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2267

R&D Systems, part of Bio-Techne
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BAF2267

Key Product Details

Validated by

Biological Validation

Species Reactivity

Feline

Applications

Flow Cytometry, Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant feline Fas/TNFRSF6/CD95
Ala25-Lys172
Accession # NP_001009314

Specificity

Detects feline Fas/TNFRSF6/CD95 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Fas and recombinant mouse Fas is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Feline Fas/TNFRSF6/CD95 Biotinylated Antibody

Detection of Fas/TNFRSF6/ CD95 antibody in Feline PBMCs treated with PMA and Ca2+ionomycin antibody by Flow Cytometry.

Detection of Fas/TNFRSF6/ CD95 in Feline PBMCs treated with PMA and Ca2+ionomycin by Flow Cytometry.

Feline peripheral blood mononuclear cells were treated with PMA and Ca2+ionomycin, then stained with Goat Anti-Feline Fas/TNFRSF6/ CD95 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2267, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Fas/TNFRSF6/CD95 antibody in Feline PBMCs by Immunocytochemistry (ICC).

Fas/TNFRSF6/CD95 in Feline PBMCs.

Fas/TNFRSF6/CD95 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline Fas/TNFRSF6/CD95 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2267) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Feline Fas/TNFRSF6/CD95 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Feline peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed feline peripheral blood mononuclear cells (PBMCs)

Western Blot

0.1 µg/mL
Sample: Recombinant Feline Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 2267-FA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Feline Fas (fibroblast associated; also named CD95 and APO-1) is a 45 kDa type I transmembrane (TM) glycoprotein that is a member of the TNF receptor superfamily (1-3). The family contains about 30 members, and is characterized by the presence of at least one cysteine-rich domain that contains multiple intrachain disulfide bonds. In general, the superfamily is divided into cytoplasmic death domain (DD) containing, and non-DD containing receptors (3). Feline Fas is synthesized as a 314 amino acid (aa) precursor that contains a 24 aa signal sequence, a 148 aa extracellular region, a 16 aa TM segment, and a 126 aa cytoplasmic tail (4). The extracellular region contains four potential N-linked glycosylation sites plus two distinct cysteine-rich domains of approximately 40 aa each; the cytoplasmic tail shows a 45 aa DD. The extracellular region of feline Fas shares 68%, 65%, 53%, and 58% aa sequence identity to porcine, human, mouse, and rat Fas, respectively. There are five alternate splice forms of feline Fas, which vary from 132 aa to 209 aa in length. All utilize exons 1-3 (aa 1-111) and all are missing the transmembrane segment of the full length form (5). Circulating Fas is reported to be both a dimer and trimer at low ng/mL concentrations. The ligand for Fas is FasL, and Fas ligation activates both the MEK cascade and FADD/caspase-8 pathway (7).

References

  1. Locksley, R.M. et al. (2001) Cell 104:487.
  2. Gaur, U. and B.B. Aggarwal (2003) Biochem. Pharmacol. 66:1403.
  3. Collette, Y. et al. (2003) Trends Immunol. 24:387.
  4. Mizuno, T. et al. (1998) Vet. Immunol. Immunopathol. 65:161.
  5. Mizuno, T. et al. (2004) Eur. J. Immunogenet. 31:159.
  6. Knipping, E. et al. (1995) Blood 85:1562.
  7. Baker, S.J. and E.P. Reddy (1998) Oncogene 17:3261.

Long Name

Fibroblast-associated

Alternate Names

Apo-1, APT1, CD95, TNFRSF6

Entrez Gene IDs

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Gene Symbol

FAS

UniProt

Additional Fas/TNFRSF6/CD95 Products

Product Documents for Feline Fas/TNFRSF6/CD95 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Feline Fas/TNFRSF6/CD95 Biotinylated Antibody

For research use only

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