FEN-1 Antibody (3H9L9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16730

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16730-100ul
NBP3-16730-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3H9L9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FEN-1 Antibodies »

Product Summary for FEN-1 Antibody (3H9L9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FEN-1 (P39748). IQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FEN-1 Antibody (3H9L9)

Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730]

Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730]

Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730] - Western blot analysis of extracts of various cell lines, using FEN-1 Rabbit mAb (NBP3-16730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730]

Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730]

Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FEN-1 Rabbit mAb (NBP3-16730) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for FEN-1 Antibody (3H9L9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FEN-1

The FEN-1 enzyme is active in base excision repair (BER) pathways. Once the DNA glycosylase removes the desired nucleotide stretch, a DNA polymerase synthesizes the complementary nucleotides for replacement. To complete the DNA repair, the FEN-1 enzyme removes the extra flap of nucleotides and DNA ligase finishes the repair.

Long Name

Flap Endonuclease 1

Alternate Names

FEN1

Gene Symbol

FEN1

Additional FEN-1 Products

Product Documents for FEN-1 Antibody (3H9L9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FEN-1 Antibody (3H9L9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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