FEN-1 Antibody (4D9)

Western Blot: FEN-1 Antibody (4D9) [NBP3-15190]

Western Blot: FEN-1 Antibody (4D9) [NBP3-15190] - Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate. All lanes: FEN-1 antibody at 0.775ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 36 KDa. Observed band size: 43 KDa.

Immunocytochemistry/ Immunofluorescence: FEN-1 Antibody (4D9) [NBP3-15190]

Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4D9) [NBP3-15190] - Immunofluorescence staining of Hela cells with at FEN-1 antibody 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: FEN-1 Antibody (4D9) [NBP3-15190]

Immunohistochemistry: FEN-1 Antibody (4D9) [NBP3-15190] - IHC image of FEN-1 antibody diluted at 1:77.5 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.