Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FEN-1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 117-380 of human FEN1 (NP_004102) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FEN-1 Antibody (OTI1F3) - Azide and BSA Free

Western Blot: FEN-1 Antibody (OTI1F3)Azide and BSA Free [NBP2-70716]

Western Blot: FEN-1 Antibody (OTI1F3)Azide and BSA Free [NBP2-70716]

Western Blot: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN-1.
Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716]

Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716]

Immunohistochemistry: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: FEN-1 Antibody (OTI1F3)Azide and BSA Free [NBP2-70716]

Western Blot: FEN-1 Antibody (OTI1F3)Azide and BSA Free [NBP2-70716]

Western Blot: FEN-1 Antibody (OTI1F3) - Azide and BSA Free [NBP2-70716] - Analysis of A549 cell lysate (35ug).
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Applications for FEN-1 Antibody (OTI1F3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FEN-1

The FEN-1 enzyme is active in base excision repair (BER) pathways. Once the DNA glycosylase removes the desired nucleotide stretch, a DNA polymerase synthesizes the complementary nucleotides for replacement. To complete the DNA repair, the FEN-1 enzyme removes the extra flap of nucleotides and DNA ligase finishes the repair.

Long Name

Flap Endonuclease 1

Alternate Names

FEN1

Gene Symbol

FEN1

Additional FEN-1 Products

Product Documents for FEN-1 Antibody (OTI1F3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FEN-1 Antibody (OTI1F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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