FEN-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84697

Novus Biologicals, part of Bio-Techne
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NBP1-84697
NBP1-84697-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FEN-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FEN-1 Antibody

Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697]

Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697]

Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining in human lymph node and kidney tissues using anti-FEN1 antibody. Corresponding FEN1 RNA-seq data are presented for the same tissues.
Western Blot: FEN-1 Antibody [NBP1-84697]

Western Blot: FEN-1 Antibody [NBP1-84697]

Western Blot: FEN-1 Antibody [NBP1-84697] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Western Blot: FEN-1 Antibody [NBP1-84697]

Western Blot: FEN-1 Antibody [NBP1-84697]

Western Blot: FEN-1 Antibody [NBP1-84697] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
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Applications for FEN-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FEN-1

The FEN-1 enzyme is active in base excision repair (BER) pathways. Once the DNA glycosylase removes the desired nucleotide stretch, a DNA polymerase synthesizes the complementary nucleotides for replacement. To complete the DNA repair, the FEN-1 enzyme removes the extra flap of nucleotides and DNA ligase finishes the repair.

Long Name

Flap Endonuclease 1

Alternate Names

FEN1

Gene Symbol

FEN1

Additional FEN-1 Products

Product Documents for FEN-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FEN-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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