Fer Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20089

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.12 mg/ml

View all Fer Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human FER (within residues 400-450). [Swiss-Prot# P16591]

Reactivity Notes

Predicted to react with mouse and canine based on 100% sequence homology.

Localization

Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fer Antibody - BSA Free

Knockdown Validated: Fer Antibody - BSA Free [NBP1-20089]

Knockdown Validated: Fer Antibody - BSA Free [NBP1-20089]

Knockdown Validated: Fer Antibody [NBP1-20089] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fer knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Fer Polyclonal Antibody (Catalog # NBP1-20089) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fer at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Fer AntibodyBSA Free [NBP1-20089]

Western Blot: Fer AntibodyBSA Free [NBP1-20089]

Western Blot: FER Antibody [NBP1-20089] - Jurkat cell lysates.
Immunocytochemistry/ Immunofluorescence: Fer Antibody - BSA Free [NBP1-20089]

Immunocytochemistry/ Immunofluorescence: Fer Antibody - BSA Free [NBP1-20089]

Immunocytochemistry/Immunofluorescence: FER Antibody [NBP1-20089] - FER antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for Fer Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Knockdown Validated

0.5 ug/ml

Western Blot

0.5 ug/ml
Application Notes
This FER antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~94 kDa. In ICC/IF cytoplasmic staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.12 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fer

FER (Feline sarcoma related) is a ubiquitously expressed non-receptor protein tyrosine kinase with about 94-kD size that acts downstream of cell surface receptors for growth factors and plays a key role in regulating actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. It acts downstream of EGFR, KIT, FCER1, PDGFRA/PDGFRB receptor etc and phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1, PTPN11, JUP, PTPN1 and STAT3. Similar to other tyrosine kinases, FER has a central Src-homology 2 (SH2) domain involved in binding to phosphotyrosine-containing peptide sequences and a highly conserved C-terminal kinase domain, whereas it is different by an NH2-terminal FER/ClP4 homology and adjacent coiled-coil domains. FER is expressed ubiquitously in human tissues and higher FER levels are observed in cancer cells compared with adjacent normal cells e.g. renal cell carcinoma.

Long Name

Feline Encephalitis Virus-related Kinase

Alternate Names

Phosphoprotein NCP94, TYK3

Gene Symbol

FER

Additional Fer Products

Product Documents for Fer Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fer Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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