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Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43584

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # F11 (HSF102)

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

The original antibody was generated by immunizing female Balb/c mice with ferritin extracted from human spleen cells. (Uniprot# Q8N4E7)

Specificity

This antibody binds human ferritin. This protein catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin mitochondrial is important for iron homeostasis.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free

Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free

Immunocytochemistry /Immunofluorescence: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] -

Immunocytochemistry /Immunofluorescence: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton, on Shi-fix coverslips stained with NBP3-43584 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic/mitochondrial staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43584, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free

Flow Cytometry: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] -

Flow Cytometry: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-43584 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Applications for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ferritin mitochondrial

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidaseactivity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation

Alternate Names

EC 1.16.3.1, ferritin H subunit, ferritin mitochondrial, ferritin, mitochondrial, mitochondrial ferritin, MtF

Gene Symbol

FTMT

Additional Ferritin mitochondrial Products

Product Documents for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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