Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43584
Recombinant Monoclonal Antibody
![Immunocytochemistry /Immunofluorescence: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] - Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free](https://resources.bio-techne.com/images/products/nbp3-43584_rb-ferritin-mitochondrial-mab-f11-hsf102-azide-bsa-free-7420252211301.png)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # F11 (HSF102)
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing female Balb/c mice with ferritin extracted from human spleen cells. (Uniprot# Q8N4E7)
Specificity
This antibody binds human ferritin. This protein catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin mitochondrial is important for iron homeostasis.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free
Immunocytochemistry /Immunofluorescence: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] -
Immunocytochemistry /Immunofluorescence: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton, on Shi-fix coverslips stained with NBP3-43584 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic/mitochondrial staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43584, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.Flow Cytometry: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] -
Flow Cytometry: Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free [NBP3-43584] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-43584 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.Applications for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ferritin mitochondrial
Alternate Names
EC 1.16.3.1, ferritin H subunit, ferritin mitochondrial, ferritin, mitochondrial, mitochondrial ferritin, MtF
Gene Symbol
FTMT
Additional Ferritin mitochondrial Products
Product Documents for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free
Product Specific Notices for Ferritin mitochondrial Antibody (F11 (HSF102)) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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