Fetuin A/AHSG Antibody (JE54-96)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32336

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32336
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE54-96

Concentration

1 mg/ml

View all Fetuin A/AHSG Antibodies »

Product Summary for Fetuin A/AHSG Antibody (JE54-96)

Immunogen

Synthetic peptide within human AHSG aa 19-68. (Uniprot: P02765)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fetuin A/AHSG Antibody (JE54-96)

Fetuin A/AHSG Antibody (JE54-96)

Western Blot: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336]

Western Blot: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336] - Western blot analysis of Fetuin A/AHSG on human placenta tissue lysates with Rabbit anti-Fetuin A/AHSG antibody (NBP3-32336) at 1/1,000 dilution.

Lysates/proteins at 20 ug/Lane.

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 5 minutes 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32336) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Fetuin A/AHSG Antibody (JE54-96)

Immunohistochemistry: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336]

Immunohistochemistry: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Fetuin A/AHSG antibody (NBP3-32336) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32336) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fetuin A/AHSG Antibody (JE54-96)

Flow Cytometry: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336]

Flow Cytometry: Fetuin A/AHSG Antibody (JE54-96) [NBP3-32336] - Flow cytometric analysis of HepG2 cells labeling Fetuin A/AHSG.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32336, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications for Fetuin A/AHSG Antibody (JE54-96)

Application
Recommended Usage

Flow Cytometry

1:500-1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fetuin A/AHSG

Human fetuin (2-Heremans-Schmid-glycoprotein or a2-HS glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver. Human fetuin is named after its bovine homolog. Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein; normal circulating levels in adults (300600 g/ml) fall significantly (3050%) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone. The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three N-linked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (N-acetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion. Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types. Fetuin plays a role as an anti-inflammatory agent by suppressing the release of TNF from stimulated macrophages. Fetuin also interacts with members of the matrix metalloprotease family of zinc dependent secreted transmembrane proteins that degrade basement membranes and extracellular matrix components. The biological activity of fetuin is mediated through its direct interaction with other proteins. Fetuin down-regulates a number of receptor tyrosine kinase family members. A motif within fetuin has homology to the TGF-b receptor type II. Circulating human plasma fetuin is partly phosphorylated which implies that phosphorylated fetuin may have a physiological function in vivo. Human fetuin isolated from plasma is a two-chain molecule consisting of a A-chain of 322 amino acid residues (53 kDa) and a B-chain of 27 residues (5 kDa). The carboxy terminal 40 amino acids of the A chain constitute a bridging peptide that may be removed proteolytically in vivo. A single mRNA encodes both the A and B chains. The apparent molecular weight of intact human fetuin is 58 kDa.

Long Name

alpha-2-HS Glycoprotein

Alternate Names

AHSG, alpha-2-HS-glycoprotein

Gene Symbol

AHSG

Additional Fetuin A/AHSG Products

Product Documents for Fetuin A/AHSG Antibody (JE54-96)

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Certificate of Analysis

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Product Specific Notices for Fetuin A/AHSG Antibody (JE54-96)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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