Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S235-22

Concentration

1 mg/ml

View all FGF-13 Antibodies »

Product Summary for FGF-13 Antibody (S235-22) - Pan Specific

Immunogen

Synthetic peptide amino acids 2-18 (AAAIASSLIRQKRQARE) of human FHF2A. 100% identical to rat, 94% identical to mouse. >80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat.

Localization

Cell Projection , Growth Cone , Dendrite , Nucleus , Cytoplasm

Specificity

Greater then 80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A. Detects approx 30kDa. Does not cross-react with FGF13B/FHF2B. Cross reacts with FGF12A/FHF1A and FGF14A/FHF4A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for FGF-13 Antibody (S235-22) - Pan Specific

Western Blot: FGF-13 Antibody (S235-22)Pan Specific [NBP2-42178]

Western Blot: FGF-13 Antibody (S235-22)Pan Specific [NBP2-42178]

Western Blot: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGF-13 (pan) protein using Mouse Anti-FGF-13 (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGF-13 (pan) Monoclonal Antibody (NBP2-42178) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.
Immunocytochemistry/ Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178]

Immunocytochemistry/ Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178]

Immunocytochemistry/Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody (NBP2-42178) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) FGFA/FHFA (pan) Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178]

Immunocytochemistry/ Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178]

Immunocytochemistry/Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cell Projection, Nucleus, Cytoplasm. Magnification: 60X.

Applications for FGF-13 Antibody (S235-22) - Pan Specific

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of FGFA/FHFA (pan) Antibody was sufficient for detection of FGFA/FHFA (pan) in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGF-13

FGF13 (fibroblast growth factor 13), belongs to the FGF family which are involved in cell growth, tissue repair, embryonic development and a number of other biological processes. FGF13 may also play a role in nervous system development/function. The location of this gene on chromosome X has been studied for links to Borjeson-Forssman-Lehmann syndrome (BFLS). FGF13 is known to have interactions with MAPK8IP2, SCN8A, CLEC3B, PRNP and EGFR.

Long Name

Fibroblast Growth Factor 13

Alternate Names

FGF13

Gene Symbol

FGF13

UniProt

AAH34340 (Human)

Additional FGF-13 Products

Product Documents for FGF-13 Antibody (S235-22) - Pan Specific

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGF-13 Antibody (S235-22) - Pan Specific

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.