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FGF-23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27553

Novus Biologicals, part of Bio-Techne
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NBP2-27553

Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-ENGYDVYLSQKHH corresponding to internal region according to NP_073148.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FGF-23 Antibody

Western Blot: FGF-23 Antibody [NBP2-27553]

Western Blot: FGF-23 Antibody [NBP2-27553]

Western Blot: FGF-23 Antibody [NBP2-27553] - Staining of Mouse Testes lysate 1 (A) and Testes lysate 2 (B) + peptide (C) (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Applications for FGF-23 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 27 kDa band observed in Mouse Testis lysates (calculated MW of 27.7 kDa according to NP_073148.1). Some lysates showed prominent bands at approx. 24+38 kDa which were successfully blocked by incubation with the immunizing peptide and have been reported in the literature (Garringer et al,. 2008, PMID:18682534).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF-23

FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.

Long Name

Fibroblast Growth Factor 23

Alternate Names

FGF23

Entrez Gene IDs

64654 (Mouse); 170583 (Rat)

Gene Symbol

FGF23

Additional FGF-23 Products

Product Documents for FGF-23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGF-23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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