FGF-4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83291

Novus Biologicals, part of Bio-Techne
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NBP1-83291
NBP1-83291-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FGF-4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGF-4 Antibody - BSA Free

Western Blot: FGF-4 Antibody [NBP1-83291]

Western Blot: FGF-4 Antibody [NBP1-83291]

Western Blot: FGF-4 Antibody [NBP1-83291] - Analysis in control (vector only transfected HEK293T lysate) and FGF4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: FGF-4 Antibody [NBP1-83291]

Immunohistochemistry-Paraffin: FGF-4 Antibody [NBP1-83291]

Immunohistochemistry-Paraffin: FGF-4 Antibody [NBP1-83291] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts and leydig cells.

Applications for FGF-4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGF-4

FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation. A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells.

Long Name

Fibroblast Growth Factor 4

Alternate Names

FGF4, HBGF-4, HST-1, KFGF, KS3

Gene Symbol

FGF4

Additional FGF-4 Products

Product Documents for FGF-4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FGF-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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