FGF basic/FGF2/bFGF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1536

Novus Biologicals, part of Bio-Techne
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NB600-1536
NB600-1536-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Bovine, Equine

Cited:

Human, Rat, Equine

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all FGF basic/FGF2/bFGF Antibodies »

Product Specifications

Immunogen

Synthetic peptide conjugated to KLH, corresponding to amino acids 1-24 of Cow FGF2.

Reactivity Notes

Equine reactivity reported in scientific literature (PMID: 24035195).

Specificity

Does not cross react with bovine FGF Acidic or bovine serum albumin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for FGF basic/FGF2/bFGF Antibody

Application
Recommended Usage

ELISA

indirect

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
Bioactivity: Anti-FGF basic (1-24) was tested in cell culture using fetal bovine heart endothelial cells (ATCC CRL 1395) plated at low cell density. Various dilutions of Anti-FGF basic(1-24) were pre incubated at 37 C with FGF basic or FGF acidic for 2 hours. Anti-FGF basic (1-24) caused a dose-dependent decrease in the mitogenic activity of FGF basic such that a final dilution of 1:50 neutralized the bioactivity of 1 unit of FGF basic.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Sterile filtered

Reconstitution

For the 0.1ml size we recommend reconstitution with 0.1ml deionized water. Rotate vial gently until powder dissolves. The 0.025ml size is provided in liquid format.

Formulation

Lyophilized from 0.2 um filtered solution in 10mM PBS (pH 7.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 54250 (Rat); 281161 (Bovine)

Gene Symbol

FGF2

UniProt

P03969

Additional FGF basic/FGF2/bFGF Products

Product Documents for FGF basic/FGF2/bFGF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FGF basic/FGF2/bFGF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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