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FGF18 Antibody (PSH01-25)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32337

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32337

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-25

Concentration

1 mg/ml

Product Summary for FGF18 Antibody (PSH01-25)

Immunogen

Recombinant protein within mouse FGF18 aa 1-207 / 207. (Uniprot: O89101)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF18 Antibody (PSH01-25)

FGF18 Antibody (PSH01-25)

Western Blot: FGF18 Antibody (PSH01-25) [NBP3-32337]

Western Blot: FGF18 Antibody (PSH01-25) [NBP3-32337] - Western blot analysis of FGF18 on different lysates with Rabbit anti-FGF18 antibody (NBP3-32337) at 1/1,000 dilution.

Lane 1: 293T whole cell lysate
Lane 2: 293T overexpress mouse FGF18 whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 15 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32337) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for FGF18 Antibody (PSH01-25)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGF-18

FGF18 (fibroblast growth factor 18), belongs to the FGF family which are involved in cell growth, tissue repair, embryonic development and a number of other biological processes. Specifically, FGF18 is crucial for the regulation of cell proliferation, cell differentiation and cell migration. FGF18 is known to have interactions with FGFR2, FGFR1, FGFR3, FGFR4 and FGF1. Disease and disorder research has been conducted in relation to FGF18 and colon cancer, leukemia, melanoma, achondroplasia, cleft lip and carcinoma.

Long Name

Fibroblast Growth Factor 18

Alternate Names

FGF18

Gene Symbol

FGF18

Additional FGF-18 Products

Product Documents for FGF18 Antibody (PSH01-25)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGF18 Antibody (PSH01-25)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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